Appendix F8 - Guidelines for Laboratory Diagnosis of SARS-CoV Infection
Supplement F: Laboratory Guidance
Public Health Guidance for Community-Level Preparedness and Response to Severe Acute Respiratory Syndrome (SARS) Version 2/3
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Laboratory confirmation of SARS-CoV infection is based on:
- Detection of any of the following by a validated test, with confirmation in a reference laboratory:
- Serum antibodies to SARS-CoV in a single serum specimen, or
- A four-fold or greater increase in SARS-CoV antibody titer between acute- and convalescent-phase serum specimens tested in parallel, or
- Negative SARS-CoV antibody test result on acute-phase serum and positive SARS-CoV antibody test result on convalescent-phase serum tested in parallel; or
- Isolation in cell culture of SARS-CoV from a clinical specimen, with confirmation using a test validated by CDC; or
- Detection of SARS-CoV RNA by RT-PCR validated by CDC, with confirmation in a reference laboratory, from:
- Two clinical specimens from different sources, or
- Two clinical specimens collected from the same source on two different days
Guidelines for the collection of specimens from potential cases of SARS are provided in Appendix F4.
Top of PageRelated Pages
- Summary
- I. Rationale and Goals
- II. Lessons Learned
- III. Diagnostic Assays
- IV. CDC's Laboratory Diagnostics Plan
- Appendix F1: Proficiency Testing for Public Health Laboratories Performing SARS-CoV EIA and RT-PCR Diagnostics
- Appendix F2: SARS-CoV Specimen Testing Guidelines: RT-PCR and Serology
- Appendix F3: Guidelines for Clinicians: The Consent Process for SARS-CoV RT-PCR and EIA Testing at CDC and Public Health Laboratories
- Appendix F4: Guidelines for Collecting Specimens from Potential SARS Patients
- Appendix F5: Laboratory Biosafety Guidelines for Handling and Processing Specimens Associated with SARS-CoV
- Appendix F6: Guidelines for Medical Surveillance of Laboratory Personnel Working with SARS-CoV
- Appendix F7: Fact Sheet for Clinicians: Interpreting SARS-CoV Test Results from CDC and Other Public Health Laboratories
- Appendix F8: Guidelines for Laboratory Diagnosis of SARS-CoV Infection
- Page last reviewed: May 3, 2005
- Page last updated: May 3, 2005
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