ELISA User's Manual
ShareCompartir
Note: Although this software and accompanying documentation is dated 2004-2005, it is still valid in 2014. Questions can be sent to CDC-INFO.
Suggested citation: Plikaytis, B.D, Carlone, G.M., Program ELISA for Windows User’s Manual, version 2. Centers for Disease Control and Prevention, Atlanta, GA, U.S.A., 2005.
U.S. DEPARTMENT OF HEALTH & HUMAN SERVICES
Public Health Service Centers for Disease Control and Prevention National Center for Infectious Diseases Division of Bacterial and Mycotic Diseases Atlanta, GA 30333
List of Chapters
- List of Figures
- Introduction
- Module 1 – Process ELISA Reader Data
- Module 2 – Template Design
- Module 3 – Abstract Data
- Module 4 – Parameter Estimation
- Module 5 – Plot Standard Curves
- Module 6 – Calculate Concentrations
- Module 7 – Edit Data Files
- Appendix A
- Appendix B
Figures
- Figure 1 — Uniform format for .DAT file required by program ELISA for Windows
- Figure 2 — File types used by program ELISA for Windows
- Figure 3 — ELISA for Windows opening menu screen
- Figure 4 — Dialog window for Module 1 – Process ELISA Reader Data
- Figure 5 — Raw data format – Dynatech MR5000 ELISA reader
- Figure 6 — Raw data format – Dynatech MR700 ELISA reader
- Figure 7 — Dialog window for Module 2 – Template Design
- Figure 8 — Display of sample template design
- Figure 9 — Dialog window for Module 3 – Abstract Data
- Figure 10 — Concluding message for Module 3 – Abstract Data
- Figure 11 — Dialog window for Module 4 – Parameter Estimation
- Figure 12 — Module 4 – ‘Pause after each iteration’ screen
- Figure 13 — Concluding message for Module 4 – Parameter Estimation
- Figure 14 — Sample output from Module 4 – Parameter Estimation
- Figure 15 — Dialog window for Module 5 – Plot Standard Curves
- Figure 16 — Typical plot of standards data overlaid with fitted standard curve
- Figure 17 — Diagram illustrating antibody concentration calculation for patient unknowns
- Figure 18 — Dialog window for Module 6 – Calculate Concentrations
- Figure 19 — Dialog window for optical density filter settings
- Figure 20 — Sample output from Module 6 – Calculate Concentrations
- Figure 21 — Logistic-log plot showing areas of antibody concentration unestimability
- Figure 22 — Sample summary (.SUM) file
- Figure 23 — Edit window from Module 7 – Edit Data Files
- Figure 24 — Display of sample template design
- Page last reviewed: September 4, 2013
- Page last updated: September 21, 2005
- Content source: