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Clinical & Laboratory Guidance

Clinical recognition of and the differences in the courses of dengue infection, the signs and symptoms and the laboratory findings are detailed in the clinical page. Below are materials for clinicians, laboratorians and epidemiologists.

Clinical guidelines for the management of dengue infection

  1. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control: WHO, 2009 [PDF – 160 pages]
  2. Transcript of Dengue Hemorrhagic Fever: early recognition, diagnosis and hospital management. An audiovisual guide for health care workers responding to outbreaks. [PDF – 14 pages].
  3. Dengue case definition
  4. CSTE Nationally Notifable Condition [PDF – 2 pages]

Clinical management tools for clinicians

  1. Poster: Chikungunya and Dengue Comparison for Healthcare Providers [PDF – 2 pages]
  2. Dengue Case Management Guide [PDF – 8 pages]
  3. Information for health care providers [PDF – 4 pages]

Dengue laboratory testing and reporting

  1. FDA approved testing for all four dengue serotypes in laboratories using the ABI7500 FAST RT-PCR [PDF – 53 pages]  and (en Español) [PDF – 56 pages]
  2. Instructions for collecting, preparing and mailing specimens for dengue testing at the CDC Dengue Laboratory (English) [PDF – 7 pages]  and (en Español) [DOC – 5 pages]
  3. Dengue Case Investigation Form (English) [PDF – 4 pages] and Dengue Case Investigation Form (en Español) [PDF – 4 pages]
  4. Hospitals, laboratories and health care centers in Puerto Rico that will draw serum samples (en Español). [DOC – 1 page]

Patient educational materials

More clinical guidance


Laboratory Guidance

Laboratory diagnosis of suspected dengue specimens involves isolation of the virus, using serological tests, or molecular methods. Detailed descriptions of the immunological response with primary and secondary infection, dengue testing algorithm for serum specimens and a description of each test.

  1. Immunological response to dengue infection
  2. Classical testing algorithms of dengue:
    1. MAC Elisa
    2. IgG Elisa
    3. Plaque Reduction and Neutralization Test (PRNT)

More laboratory guidance


Differentiating Chikungunya From Dengue: A Clinical Challenge
Medscape Commentary, Dr. Tyler Sharp, CDC