Identification of other Streptococcus Species: Streptococcus General Methods

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The reference used for compiling the methods in Section I is:
Murray, P.R., Baron, E. J., Jorgensen, J.J., Pfaller, M.A., and Yolken, R.H. Manual of Clinical Microbiology, 8^th ed. ASM Press: Washington, DC, 2003.

The Streptococcus species identification methods in Section II were compiled by several individuals, including Lynn Shewmaker, Richard Facklam, and Lucia Teixeira.

Section I.

1. Accuprobe-Enterococcus Test
2. Accuprobe-Pneumococcus Test
3. Acid formation in carbohydrate broth
4. Arginine Hydrolysis
5. Bacitracin Test
6. Bile-esculin Test
7. Bile solubility Test
8. CAMP Test
9. Catalase Test
10. Clindamycin test
11. Esculin hydrolysis
12. Gas from MRS broth
13. Gram Stain
14. Growth at 10 & 45C
15. Hemolysis
16. Hippurate hydrolysis
17. Lancefield Group Antigen
18. Leucine amino peptidase (LAP)
19. Litmus Milk Test
20. Motility
21. 6.5% NaCl Tolerance Test
22. Optochin
23. Pigmentation
24. Pyridoxal Requirement Test (Vitamin B6)
25. Pyrrolidonlarylamindase (PYR)
26. Pyruvate Utilization Test
27. Satellite Test
28. Starch hydrolysis
29. Sucrose agar (extracellular polysaccharides)
30. Sucrose broth (5%)
31. Tellurite tolerance
32. Urea hydrolysis
33. Vancomycin test
34. Voges-Proskauer

Section II.

Identification Procedures for Catalase-Negative Gram-positive Cocci

A. General Description of the general identified in this lab

Table 1.

Identification of gram-positive general Beta-hemolytic streptococci

Table 2.

Identification and reporting of β-hemolytic streptococci

Table 3.

Identification of atypical group A strep

Table 4.

Identification of the S. anginous group
Non-beta-hemolytic streptococci
Streptococcus pneumoniae

Table 5.

Identification non-β-hemolytic strep

Table 6.

Characteristics of viridans streptococci

Table 7.

Identification of nutritionally variant strep

B. Identification of Enterococcus and Vagococcus

Table 8.

Identification of Enterococcus and Vagococcus species

C. Identification of Lactococcus species

Table 9.

Identification of Lactococcus species

D. Identification of Gemella species

Table 10.

Identification of Gemella species

E. Identification of Pediococcus and Tetragenococcus species

Table 11.

Identification of Pediococcus and Tetragenococcus

F. Identification of Leuconostoc species

Table 12.

Identification of Leuconostoc species

G. Identification of Aerococcus and Helococcus

Table 13.

Identification of Aerococcus and Helococcus

H. Identification of Globicatella

Table 14.

Identification of Globicatella

I. Identification of Dolosigranulum, Ignavigranum, Facklamia, and Alloicococcus

Table 15.

Identification of Dolosigranulum, Ignavigranum, Facklamia, and Alloicococcus

Table 16.

Rapid ID-32 for unusual gram positive bacteria