Protocols
ShareCompartir
Because of differences in the bacteria that PulseNet subtypes, PulseNet has developed separate protocols for each bacteria that are subtyped by PFGE, MLVA, and WGS. Additionally, because of the variation between machines, MLVA protocols have been developed separately for specific machines. These protocols are listed below and can also be found on CDC’s Web pages describing each pathogen.
PFGE Protocols
MLVA Protocols
- Analysis: Shiga-Toxin Producing E. coli O157 Applied BioSystems Genetic Analyzer 3130/3500 [PDF – 10 pages]
- Analysis: Shiga-Toxin Producing E. coli O157 Beckman Coulter CEQ 8000/8800/GeXP [PDF – 10 pages]
- Analysis: Salmonella Enteritidis Beckman Coulter CEQ 8000/8800/GeXP [PDF – 11 pages]
- Analysis: Salmonella Enteritidis Applied BioSystems Genetic Analyzer 3130/3500 [PDF – 10 pages]
- Analysis: Salmonella Typhimurium Applied BioSystems Genetic Analyzer 3130/3500 [PDF – 9 pages]
- Analysis: Salmonella Typhimurium Beckman Coulter CEQ 8000/8800/GeXP [PDF – 10 pages]
- Laboratory Procedure: Shiga-Toxin Producing E. coli O157 Applied Biosystems Genetic Analyzer 3130XL [PDF – 18 pages]
- Laboratory Procedure: Shiga-Toxin Producing E. coli O157 Applied Biosystems Genetic Analyzer 3500 Platform [PDF – 20 pages]
- Laboratory Procedure: Salmonella Enteritidis Applied Biosystems Genetic Analyzer 3130XL Platform [PDF – 18 pages]
- Laboratory Procedure: Salmonella Enteritidis Applied Biosystems Genetic Analyzer 3500 Platform [PDF – 20 pages]
- Laboratory Procedure: Salmonella Enteritidis Beckman Coulter CEQ 8000/8800/GeXP Platform [PDF – 17 pages]
- Laboratory Procedure: Salmonella Typhimurium Applied Biosystems Genetic Analyzer 3130XL Platform [PDF – 18 pages]
- Laboratory Procedure: Salmonella Typhimurium Applied Biosystems Genetic Analyzer 3500 Platform [PDF – 20 pages]
- Laboratory Procedure: Salmonella Typhimurium Beckman Coulter CEQ 8000/8800/GeXP Platform [PDF – 17 pages]
WGS Protocols
*Clostridium botulinum is considered a select agent and, as such, follows different handling procedures than other pathogens covered by PulseNet.
- Page last reviewed: February 25, 2016
- Page last updated: February 25, 2016
- Content source: