PulseNet Methods

PulseNet uses a variety of methods to subtype: E. coli (O157 and other Shiga toxin-producing E. coli), Campylobacter, Listeria monocytogenes, Salmonella, Shigella, Vibrio cholerae, Vibrio parahaemolyticus, and Cronobacter isolates. Pulsed-field gel electrophoresis (PFGE), multiple locus variable number tandem repeat analysis (MLVA),and whole genome sequencing (WGS)  are PulseNet’s main subtyping (or fingerprinting) tools.