Acholeplasma laidlawii

Acholeplasma laidlawii are small bacteria which lack a cell wall.[2] Like other Acholeplasma and Mycoplasma, A. laidlawii has been identified as a common contaminant of growth media for cell culture.[3]

Acholeplasma laidlawii
Scientific classification
Kingdom:
Bacteria
Division:
Firmicutes
Class:
Mollicutes
Order:
Acholeplasmatales
Family:
Acholeplasmataceae
Genus:
Acholeplasma
Binomial name
Acholeplasma laidlawii
(Sabin 1941) Edward and Freundt 1970[1]

History

A. laidlawii was first isolated from sewage in London in 1936 and was named after its discoverer, Patrick Laidlaw.[4]

Genetics

A. laidlawii has a relatively small genome comprising 1.5Mbp. Additionally its genome has a low GC-content of just 31%.[2] The A. laidlawii genome has been sequenced.[2]

In Research

Acholeplasma laidlawii may contaminate bovine serum and also occurs in serum-free cell culture media products. The presence of A. laidlawii in broth powders is a serious problem in routine biopharmaceutical operations where filtration is used as a sterilisation procedure. A. laidlawii may flourish and survive for prolonged periods at refrigeration and ambient temperatures in serum-free cell culture media.

References
  1. Parte, A.C. "Acholeplasma". www.bacterio.net.
  2. Lazarev VN; Levitskii SA; Basovskii YL; Chukin MM; Akopian TA; et al. (September 2011). "Complete genome and Proteome of Acholeplasma laidlawii". J. Bacteriol. 193 (18): 4943–4953. doi:10.1128/jb.05059-11. PMC 3165704. PMID 21784942.
  3. Windsor HM; Windsor GD; Noordergraaf JH (March 2010). "The growth and long term survival of Acholeplasma laidlawii in media products used in biopharmaceutical manufacturing". Biologicals. 38 (2): 204–210. doi:10.1016/j.biologicals.2009.11.009. PMID 20153666.
  4. Laidlaw PP; Elford WJ (2 June 1936). "A New Group of Filterable Organisms". Proceedings of the Royal Society of London. 120 (818): 292–303. doi:10.1098/rspb.1936.0036. JSTOR 82051.
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