Total complement activity
Total complement activity is a test performed to assess the level of functioning of the complement system.
|C4 (C)||FB (A)||C3||CH50||Conditions|
|·||↓||↓||↓||PSG, C3 NeF AA|
|Total complement activity|
The terms "CH50" or "CH100" may refer to this test. The test is based on the capacity of a serum to lyse sheep erythrocytes coated with anti-sheep antibodies (preferably rabbit IgG).
In combination with the Alternative pathway hemolytic assay ("AH50") it can indicate terminal pathway deficiencies (C3, C5-C9; absence of hemolysis in both CH50 and AH50), classic pathway deficiencies (C1, C2, C4; absence of lysis in CH50) and alternative pathway deficiencies (Factor I, B, H, D, properdin; absence of lysis in AH50).
- Michael T. Lotze; Angus W. Thomson (2005). Measuring immunity: basic biology and clinical assessment. Academic Press. pp. 151–. ISBN 978-0-12-455900-4. Retrieved 3 December 2010.
- "Complement: MedlinePlus Medical Encyclopedia". medlineplus.gov. Retrieved 11 February 2017.