Immunodiffusion is a diagnostic test which involves diffusion through a substance such as agar which is generally soft gel agar (2%) or agarose (2%), used for the detection of antibodies or antigen.

Medical diagnostics

The commonly known types are 1. Single diffusion in one dimension (Oudin procedure) 2. Double diffusion in one dimension (Oakley Fulthorpe procedure) 3. Single diffusion in two dimension (radial immunodiffusion or Mancini method) 4. Double diffusion in two dimensions (Ouchterlony double immunodiffusion) .[1]


  1. (1) "Immunodiffusion". ScienceDirect. Elsevier B.V. Archived from the original on 2017-05-02. Retrieved 2017-05-19.
    (2) "Ouchterlony double immunodiffusion" (photograph). Retrieved 2017-05-15.
    (3) "Radial Immunodiffusion". Edvotek, Inc. 2017. Archived from the original (photograph) on 2017-08-07. Retrieved 2017-08-07. Photograph of precipitin circles in a Petri dish during radial immunodiffusion.
  • Immunodiffusion at the US National Library of Medicine Medical Subject Headings (MeSH)
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