Bacterial capsule

It usually consists of polysaccharides,[4] but can be composed of other materials such as glycoprotein, polypeptide D-glutamic acid in B. anthracis, and peptidoglycan and muramic acid found in E.coli bacterial capsule. Because most capsules are so tightly packed, they are difficult to stain because most standard stains cannot adhere to the capsule. For examination under the microscope, the bacteria and their background are stained darker than the capsule, which doesn't stain. When viewed, bacterial cells as well as the surface they are on, are stained dark, while the capsule remains pale or colorless and appears as a ring, or halo, around the cell.[5]

The capsule is considered a virulence factor because it enhances the ability of bacteria to cause disease (e.g. prevents phagocytosis). The capsule can protect cells from engulfment by eukaryotic cells, such as macrophages.[6] A capsule-specific antibody may be required for phagocytosis to occur. Capsules also contain water which protects the bacteria against desiccation. They also exclude bacterial viruses and most hydrophobic toxic materials such as detergents. Immunity to one capsule type does not result in immunity to the other types. Capsules also help cells adhere to surfaces. As a group where the capsule is present they are known as polysaccharide encapsulated bacteria or encapsulated bacteria.[7]

The capsule is found most commonly among gram-negative bacteria:

• Escherichia coli (in some strains)
• Neisseria meningitidis[8][9][10]
• Klebsiella pneumoniae[11][12][13]
• Haemophilus influenzae[14]
• Pseudomonas aeruginosa[15]
• Salmonella[16]

However, some gram-positive bacteria may also have a capsule:

• Bacillus megaterium for example, synthesizes a capsule composed of polypeptide and polysaccharides.
• Streptococcus pyogenes synthesizes a hyaluronic acid capsule.
• Streptococcus pneumoniae[17] has at least 91 different capsular serotypes.[18] These serotypes are the basis for the pneumococcal vaccines.
• Streptococcus agalactiae produces a polysaccharide capsule of nine antigenic types that all contain sialic acid (Ia, Ib, II, III, IV, V, VI, VII, VIII).
• Staphylococcus epidermidis
• Staphylococcus aureus

The yeast Cryptococcus neoformans,[19] though not a bacterium, has a similar capsule.[20][21]

Capsules too small to be seen with an ordinary microscope, such as the M protein of Streptococcus pyogenes, are called microcapsules.

1. India ink staining: the capsule appears as a clear halo around the bacterium as the ink can't penetrate the capsule.[22]^:87
2. Maneval's capsule stain: the capsule appears as a clear halo between the pink-stained bacterium and the bluish-grey stained background. The background stain is the acidic stain Congo red (which changes color to bluish-grey due to the pH), and the pink stain is acid fuschin.
3. Serological methods: Capsular material is antigenic and can be demonstrated by mixing it with a specific anticapsular serum. When examined under the microscope, the capsule appears 'swollen' due to an increase in its refractivity. This phenomenon is the basis of quellung reaction.

Vaccination using capsular material is effective against some organisms (e.g., H. influenzae type b,[23][24] S. pneumoniae, and N. meningitidis[25]). However, polysaccharides are not highly antigenic, especially in children, so many capsular vaccines contain polysaccharides conjugated with protein carriers, such as the tetanus toxoid or diphtheria toxoid. This stimulates a much more robust immune response.[26]

• Bacterial cell structure
• Quellung reaction, a method to visualize capsule under a microscope